Microbiology lecture notes: Bacterial Metabolism continued......

* We will continue talking about bacterial metabolism (see Sept. 10th lecture notes for introduction to bacterial metabolism)

Anaerobic growth is very slow  (One glucose: 2 ATPs)
Aerobic respiration allows faster growth  (One glucose: 38 ATPs)

Aerobic Respiration
Krebs cycle + Electron transport chain + Chemiosmosis + Oxidative Phosphorylation

1) Krebs Cycle: Pyruvate converted to acetyl-CoA, oxidized in cycle
    -In prokaryote cytoplasm
    -Generates CO2, some ATP
    -Most important: Generates NADH, FADH2 reducing potential

2) Electron Transport:
    -At cell membrane
    -NADH, FADH2 pass electrons via carriers to O2
    -H+ pumping creates gradient

3) Chemiosmosis: Proton gradient provides energy for ATP synthesis by oxidative phosphorylation

    Review Tables 5.2 and 5.3, p. 125

Alternative energy sources: Lipids, Proteins
1) Lipids hydrolyzed to acetyl-CoA, fed into Krebs cycle
2) Proteins hydrolyzed to amino acids, deaminated, fed into glycolysis or Krebs cycle

Amphibolic pathways: Catabolic pathways provide intermediates for anabolic synthesis pathways and vice versa