B.S. Microbiology The University of Michigan, Ann Arbor 1984
Ph.D. Molecular Immunology Department
of Molecular Genetics and Biochemistry
1991
University of Pittsburgh School of Medicine
Postdoctoral Fellowship
Zentrum für Molekulare Biologie
1991-1993
University of Heidelberg, Germany
Postdoctoral Fellowship
Seattle Biomedical Research Institute
1994-1997
University of Washington
Associate Scientist
Seattle Biomedical Research Institute
1997-1999
University of Washington
Assistant Professor of Biology Concordia College 1999-present
Graduate Research/Teaching Assistant,
Department of Molecular Genetics and Biochemistry,
The University of Pittsburgh School of Medicine, Pittsburgh, PA
1984-1991
Thesis title: “Transcription termination in the mouse immunoglobulin
gamma
2a and gamma 2b heavy chain genes”
Thesis advisor: Christine Milcarek, Ph.D.
Investigated developmental transcriptional regulation in the immunoglobulin
heavy chain genes using mouse tumor cell models.
Postdoctoral Fellow,
Zentrum für Molekulare Biologie,
University of Heidelberg, Heidelberg, Germany
1991-1993
Advisor: Christine Clayton, Ph.D.
Studied developmental transcriptional regulation of surface coat protein
gene expression in the African parasite Typanosoma brucei.
Postdoctoral Fellow,
Seattle Biomedical Research Institute, Seattle, Washington
1994-1997
Postdoctoral Scholar,
Department of Pathobiology, School of Public Health and
Community Medicine, University of Washington
1994-present
Advisor: Marilyn Parsons
Identified and characterized genes involved in biogenesis of the glycosome
organelle in pathogenic Leishmania parasites.
Associate Scientist
1997-present
Seattle Biomedical Research Institute, Seattle, Washington
Identification and characterization of genes involved in biogenesis
of the glycosome organelle in pathogenic Leishmania parasites. Project
management and supervision.
1985-1990 Taught Medical Microbiology laboratory course,
University of Pittsburgh
School of Medicine
1989-1990 Provided lectures in undergraduate Immunology course, University of Pittsburgh School of Medicine
1992-1993 Taught undergraduate Molecular Biology laboratory
course in both
German and English, University of Heidelberg, Germany
1994, 1999 Provided lectures in protozoan cellular
biology as part of Advanced
Biochemistry course, Seattle Pacific University
1998 Provided lecture in Molecular Parasitology course, University of Washington
1999
Guest lecturer in Control of Eukaryotic Gene Expression course.
Universidad Central de Venezuela, Caracas, Venezuela
1999 Taught general Microbiology lecture/laboratory course at Seattle Central Community College
1999-present Vertebrate Biology, Microbiology and Immunology/Parasitology,
Concordia College, Moorhead MN
Informal Teaching/Training
1987-1991 Supervised one undergraduate student
researcher and one graduate student
researcher in independent research projects, University of Pittsburgh
School of Medicine
1988-1990 Advised graduate students as president
of Biomedical Graduate Student
Organization, University of Pittsburgh School of Medicine
1994-1999 Trained and supervised two undergraduate
students in independent research
projects, trained and supervised three research associates and one masters’
student in independent research projects, Seattle Biomedical Research
Institute
1999-present Summer undergraduate research, Concordia College,
Moorhead, MN
Flaspohler, J.A. and C. Milcarek. 1990. Myelomas and lymphomas expressing the Ig gamma 2a heavy chain gene have similar transcription termination regions. J. Immunol. 144:2802-2810.
Flaspohler, J.A. and C. Milcarek. 1992. Use of In Vitro-transcribed RNAs as immobilized targets during RNA:RNA hybridization. BioTechniques 13(1):68-72.
Flaspohler, J.A., D. Boczkowski, B.L. Hall, and C. Milcarek. 1995. The 3’-untranslated region of membrane exon 2 from the gamma 2a immunoglobulin gene contributes to efficient transcription termination. J. Biol. Chem. 270(20):11903-11911.
Biebinger, S., S. Rettenmaier, J. Flaspohler, C Hartmann, J. Peña-Diaz, L.E. Wirtz, H.R. Hotz, J.D. Barry, and C. Clayton. 1996. The PARP promoter of Trypanosoma brucei is developmentally regulated in a chromosomal context. Nucleic Acids Res. 24(7):1202-1207.
Flaspohler, J.A., W.L. Rickoll, S.M. Beverley, and M. Parsons. 1997. Functional identification of a Leishmania gene related to peroxin 2 reveals common ancestry of glycosomes and peroxisomes. Mol. Cell. Biol. 17(3):1093-1101.
Hotz, H.R., S. Biebinger, J. Flaspohler, and C. Clayton. 1998. PARP gene expression: control at many levels. Molec. Biochem. Parasitol. 91(1):131-143.
Flaspohler, J.A., K. Lemley, and M. Parsons. 1999. A dominant negative mutation in the GIM1 gene of Leishmania donovani is responsible for defects in glycosomal protein localization. Molec. Biochem. Parasitol. 99(1):117-128.
Mannion-Henderson, J., J.A. Flaspohler, K.R. Lemley, W.L. Rickoll and M. Parsons. 2000. Isolation and characterization of Leishmania mutants defective in glycosomal protein import. Mol. Biochem. Parasitol. 106(2): 225-237.
Flaspohler, J.A. and C. Milcarek (1986) Mapping the transcription termination
region of the murine immunoglobulin gamma 2b gene. Presented at the 26th
annual meeting of the American Society for Cell Biology:167, Washington
D.C.
Flaspohler, J.A. and C. Milcarek (1988) Transcription termination in
the murine immunoglobulin gamma 2b and gamma 2a genes. Presented at RNA
Processing meeting:56, Cold Spring Harbor, NY.
Flaspohler, J.A. and C. Milcarek (1990) Transcription termination and
the regulation of murine immunoglobulin gamma 2a and gamma 2b gene expression.
Presented at RNA Processing meeting:91, Cold Spring Harbor, NY.
Flaspohler, J.A. and C. Milcarek. (1991) Transcription termination
and poly(A) site usage in the murine gamma 2b and gamma 2a heavy chain
genes. Presented at the 20th annual Transcription Initiation and
Elongation meeting, Keystone symposium, Keystone, CO. J. Cell. Biochem.
Suppl. 1991. 15G:235.
*Janz, L., J. Flaspohler, M. Hug, and C. Clayton (1991) Characterization
of the promoters for major surface protein transcription in trypanosomes.
Presented at Molecular Parasitology meeting II:2, Woods Hole, MA.
Flaspohler, J.A., S. Biebinger, C. Hartmann, B. Paust, S. Rettenmaier,
H.R. Hotz, L. Wirtz, J. Barry and C. Clayton. (1995) Developmental regulation
of surface antigen gene transcription in T. brucei. Molecular Parasitology
meeting VI:24, Woods Hole, MA.
*Flaspohler, J.A., S. Beverley and M. Parsons (1995) Identification
of a L. donovani sequence which rescues a cell line defective in glycosomal
compartmentalization. Presented at Molecular Parasitology meeting VI, Woods
Hole, MA.
*Flaspohler, J.A., K. Radford, S. Beverley, and M. Parsons (1996) Glycosome
biogenesis: Complementation analysis identifies a Leishmania sequence
homologous to peroxisome assembly factor 1. Presented at Molecular Parasitology
meeting VII:6, Woods Hole, MA.
*Flaspohler, J.A, K. Radford, W. Rickoll and M. Parsons (1997)
A mutation in the GIM1 gene: A dominant negative role in glycosome biogenesis?
Presented at Molecular Parasitology meeting VIII, Woods Hole, MA.
Flaspohler, J.A., K. Lemley, J. Stevens, and M. Parsons (1998)
Characterization of a dominant negative mutation affecting glycosome biogenesis
in Leishmania donovani. Presented at Molecular Parasitology meeting
XI, Woods Hole, MA.
Parsons, M., J. Mannion-Henderson, J. Stevens, and J. Flaspohler (1999)
Genetic approaches to Leishmania cell biology and biochemistry. Presented
at International training and research in emerging infectious diseases
Asian workshop on Intracellular Pathogens, Jawaharlal Nehru University,
New Delhi, India
* Indicates abstract presented as plenary talk
Flaspohler, J.A., N. Cochran and M. Parsons (1994) Towards identification
of genes involved in glycosome biogenesis in L. donovani. Presented at
Seattle Protozoology conference, Seattle, WA.
Flaspohler, J.A., K. Radford, S. Beverley, and M. Parsons (1996) Identification
of a Leishmania DNA sequence which rescues a L. donovani cell line defective
in glycosomal biogenesis. Presented at Seattle Protozoology conference,
Seattle, WA.
Flaspohler, J.A., K. Radford, W. Rickoll, S. Beverley, and M. Parsons
(1997) The GIM1 gene of Leishmania donovani is homologous to the peroxin
2 family of proteins and represents the first identified gene involved
in glycosome biogenesis. Presented at Seattle Protozoology conference,
Seattle, WA.
Flaspohler, J.A., J. Mannion, J. Stevens, C. Crudder and M. Parsons
(1999) The GIM1 gene of Leishmania donovani: Involvement in glycosome import/biogenesis.
Presented at Seattle Protozoology conference, Seattle WA.
Further oral presentations are indicated by * in Abstracts section
above
1999 Seminar entitled “Glycosome biogenesis in Leishmania donovani”
presented at the Universidad de Caracas and the Universidad de los Andes,
Merida, Venezuela
2000 Seminar entitled “Genetic analysis of organelle biogenesis in
the parasitic protozoan Leishmania donovani” presented at the Microbiology
Department, North Dakota State University, Fargo, ND, USA
2001 Seminar entitled “A human parasite of worldwide importance: Genetic
dissection of organelle biogenesis in Leishmania donovani” presented
at the Biology department, Moorhead State University, Moorhead MN, USA
2001 Seminar “The enemy within: Genetic studies on Leishmania:
A parasitic pathogen of humans worldwide.” Presented a Concordia College
Faculty Colloquia series.
1984-1991 University of Pittsburgh School of Medicine Department
of Molecular
Genetics and Biochemistry Dean’s Fellowship/Teaching Assistant Fellowship
1996-1999 Awarded 3-year National Research Service Award Postdoctoral
Fellowship
for proposal entitled “Glycosome deficiency and biogenesis in Leishmania
donovani”
Fellowship #1 F32 AI09318-01A1
1996 Kenneth Warren
award for outstanding presentation by a young
investigator at Molecular Parasitology meeting VII, Woods Hole, MA